The RNAs at cellular level

ImaBiotech offers a unique solution to map the  RNAs therapeutics and Whole Transcriptome Atlas at cellular from FFPE or Frozen tissues using different technologies such as GeoMx Nanostring or RNAscope.

With full coverage of protein coding genes, WTA services delivers spatial analysis of any target in any tissue in the biological region that matter most. This technology delivers the maximum amount of sensitivity and can be customized with up to 60 additional targets to assay non-coding RNA, synthetic DNA or exogenous genes.

GeoMx WTA comes along with Illumina library prep protocols and streamlined NGS analysis pipelines that allows to explore pathways across the whole transcriptome in user defined regions of interest and measure the impact of a drug or a disease at the cellular level. ImaBiotech developed routine analysis in GLP environment for many applications in Oncology (Learn more), CNS (Learn more) or Cardio-Metabolic disorders (Learn more)

Integrated in ImaLink platform, WTA and ImaBiotech Capabilities provides unique tools to measure, quantify DNA, ARN, Virus and Drug distribution simultaneously to better understand disease and drug efficacy.

LMD coupled to Ncounter for rapid targeted RNA targets

From a tissue section, regions of interest (such as stroma, necrotic tissue, inflammations, cancer cells) are selected and micro dissected using LMD Leica system (learn more). Each pooled cells or regions are diluted in solution in order to extract RNA. Using Nanostring Ncounter technology or Illumina RNA sequencing tools, we amplify and quantify the expression of genes in particular regions. 

RNAscope® Technology is a cutting-edge in situ hybridization (ISH) technology enabling RNA target expression analysis.

The RNAscope® Assay detect mRNA, miRNA and ncRNA targets of >300bp within intact cells and tissues with high sensitivity and specificity. The proprietary probe design requires an average of 20 Z pairs per target to visualize mRNA transcript for cytokines, transcription factors, and low expressing targets.

Integrated in ImaLink platform, RNAscope provides an unique tool to identify at the level of single molecule, a target of interest that could guide the high-plex profiling.


  • Spatial Disease Analysis through gene expression of specific cells
  • Correlation of Drug exposure to cell and impact to gene expression
  • Biomarker discovery/validation in specific cells
  • Correlation gene expression and protein levels in tissues


PublicationHigh-Plex Predictive Marker Discovery for Melanoma Immunotherapy-Treated Patients Using Digital Spatial Profiling
PublicationDigital quantitative assessment of PD-L1 using digital spatial profiling
PublicationBiomarkers Associated with Beneficial PD-1 Checkpoint Blockade in Non-Small Cell Lung Cancer (NSCLC) Identified Using High-Plex Digital Spatial Profiling
ArticleMethod of the Year 2020: spatially resolved transcriptomics
BrochureGeoMx Whole Transcriptome Atlas
White Paper Spatially resolved tumor gene expression analysis identifies SERPINE1 as a molecular switch of CRC progression

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