Come and meet Dorothy Zelent, Lauranne Poncelet (PhD student) at ImaBiotech’s Booth 111 at SID 2019 in Chiacgo (USA).
Be one of the five first registrants and obtain a FREE WINE SET.
You will be able to discover our latest work in dermatology with our oral presentation and poster by Lauranne Poncelet in partnership with: Sorbonne Universities, University of Lille, Hospital Tenon in Paris, Hospital Saint Louis in Paris, CEA, Clinical Research Center for Hair and Skin Science.
Title: Identification of biomarkers of early innate events during skin reaction following intradermal injection
Session Category: Concurrent 11
Session Name: Pharmacology and Drug Development
Session Order: CM11-03
Session Date and Time: May 10, 2:45 – 5:15 PM
Location: Hilton Chicago, 720 South Michigan Avenue, Chicago, Illinois, 60605, USA
Abstract: The skin as the interface with the external environment plays a crucial role in host defense against microbial attack. Skin innate cells must provide sufficient information to immune system to organize host defenses against invaders. Using this unique feature, skin has become a promising site for vaccine administration. Richness in antigen-presenting cells and inflammatory molecules provided by skin tissue provide sufficient information to mount an adaptive immunity. However, early innate molecular events are poorly understood. We aim to determine novel biomarkers of skin reaction following intradermal (i.d.) immunization. We used an ex vivo human explant model from healthy donors and investigated by Nano LC-MS/MS analysis, MALDI-MSI imaging to improve our comprehension of early innate molecular events produced at the inoculation site following i.d. Influenza trivalent vaccine (TIV) administration. In this proof-of-concept study, we identified early innate immune proteins differentially expressed in dermal and epidermal layer at 4 to 18 hours following immunization compared to baseline. Top network of proteins that were up-regulated in the dermis were related to cell-to-cell signaling and cell trafficking, whereas in the epidermis top protein networks were associated with antigen-presenting cells at early time point following immunization. In the dermis, detected signature were associated with chemokines such as CXCL8 that can attract neutrophils following dermal immunization. In the epidermis, top networks are linked to CXCL10, which is detected by qPCR as markers of antigen-presenting cell activation. In addition, Maldi-MSI imaging of the skin allowed to localize in the tissue major metabolites related to these signatures. Our study proposes novel step-forward approach for the identification of biomarkers during early innate reaction at the site of immunization that could correlate with immune protection.