Protein expression at the cellular level!
Understanding tissue heterogeneity is critical to answering key biological questions and a key concept in identifying clinically relevant association. ImaBiotech developed routine analysis of innovative high multiplex immuno-staining services in GLP environment for many applications in Oncology (Learn more), CNS (Learn more) or Cardio-Metabolic disorders (Learn more) with a unique testing panels (Create your panel)
Our Different Techniques
GeoMx DSP uses fluorescently-labeled antibodies to define a region of interest (ROI). Antibodies labeled with unique photocleavable oligonucleotide are also applied on a 5 micron tissue section. Every selected region of interest is sequentially exposed to UV light to decouple the oligonucleotides from the profiling reagents. Decoupled oligonucleotides deposited into wells are hybridized to Nanostring® barcodes and quantitated on the nCounter platform.
This non invasive approach allows the preservation of the tissue integrity, captures the tissue morphology and permits the high multiplexing of protein quantification – up to 80 proteins in a run from a single tissue section.
Hyperion™ Imaging System (Imaging Mass Cytometry technique) developed by Fluidigm Corp allows the analysis and quantification of 40+ markers simultaneously from a single slide. The second advantage of Imaging Mass Cytometry compared to fluorescent microscopy is that there is almost no noise in the data as each metal isotopes has its on distinct detection peak and does not overlap with other metal isotopes. There is almost zero background noise and thus the contrast between markers of interest and the background is ideal for image analysis.
- Spatially differentiated profiling of tumor driver and immune-oncology proteins (Learn more) and CNS (Learn more)
- Biomarker discovery related to location and signaling of specific cells
- Pathways analysis across tissue structures and complexity
- High dimensional phenotypic and functional analysis of single cells