Mass spectrometry Imaging brain images analyses drug efficacy

Mass Spectrometry Imaging: Has Never Been So Close to You

MSI

Objective:

In this application note, we are going to describe our new cloud platform: Multimaging TM. This platform enables rapid analysis and sharing of mass spectrometry imaging data.

This technology is based on:

  •  A cloud software to store, share and analyse large data sets (>1 Terabyte)
  • The use of the histology, pathology and immunoassay data in order to deepen and validate the first analyses made by Mass Spectrometry Imaging
  • An innovative use of quality controls ensuring effective standardization of data extracted from multiple MSI acquisitions
  • The integration of statistical tools allowing the detailed analysis of images in order to discover new biomarkers

Context:

All -omic studies require managing the quality and the variability of large cohorts of samples to provide reliable interpretations.
A typical imaging study to discover biomarkers needs hundreds of gigabytes of data combining large datasets (20 to 200 of images) with high spectral and spatial resolution (up to 30k positions/images).

Today, most sofware goes through an initial memory loading step which limits the use of
large cohorts. Some of them do not allow the simultaneous use of multiple images. Others
allow the loading of several images at the same time but statically.

Generally, this amount of data brings issues relating to sharing. One of the next
future challenges in MSI will be to share these large data as quick as possible.

Solution:

Our strategy was to develop a platform with a BigData architecture using quality controls or internal standards to manage the reproducibility of several large datasets obtained from different acquisitions (Figure 1) .

The innovative architecture allows us to  visualize and to analyze a project with a  numerous images in a few minutes. (Figure 2)

Multimaging allows you to overlay  molecular distributions with high-definition
images by using traditional formats like TIFF or JPEG but also proprietary formats like
Mirax, Aperio or Hamamatsu. (Figure 3) This  high definition brings a great advantage in the biological interpretation of molecular responses.

Multimaging also includes a statistical module for biomarker research by using large
cohorts of region of interest to obtain statistically reliable results.

Multimaging allows the ease of instantly  sharing data globally between subsidiaries
and collaborators. Using cloud architecture,  this allows ImaBiotech to provide the  distribution and analysis results remotely  without having to download the entire data set.

Summary:

Multimaging allows the simultaneous management and analysis of several mass spectrometry imaging datasets in a short period of time.

This platform allows the management of quality controls to take into account the inter and intra variability between samples.

Based on the combination of the molecular distribution and high resolution histological staining, potential biomarkers and their specific tissue distribution can be confirmed.

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