Multimaging_HD

MALDI Imaging of Small Histological Tissue Applied to Ophthalmology: Qualitative and Quantitative Approach in Drug Discovery

Gregory Hamm (1); Nicolas Desbenoit (2, 6); Legouffe Raphael (1); Fabien Pamelard (1); David Bonnel (1); Olivier Laprevote3, 7; Alain Brunelle3; Maxence Wisztorski (5); Jean-Pierre Both (4); Christophe Baudoin (2, 6); Françoise Brignole-Baudouin (2, 6); Jonathan Stauber (1)

1 ImaBiotech, MS Imaging Department, Lille, France;
2 Institut de la Vision, INSERM, UMR_S968, Paris, France;
3 Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France;
4 CEA-LIST, Gif-sur-Yvette, France;
5 FABMS, Université¸ de Lille, Villeneuve d’Ascq, France;
6 CHNO des Quinze-Vingts, Paris, France;
7 CTAC, Université Paris Descartes, Paris, France

Abstract
Mass Spectrometry Imaging (MSI) has become a common technique to detect the localization of molecules directly on the surface of biological tissues without labeling. Recently, numerous studies have dealt with the growing interest in combining quantitative and distribution analyses using MSI. This application may play a significant role in early phases of pharmaceutical discovery to evaluate small molecule concentration, notably drugs. Moreover, unlike classical imaging and quantification techniques, MSI can provide precise and selective quantitative information at micrometer level to differentiate fine histological structures. Our approach, first described on model tissues, will then be applied in an ophthalmic study to assess the distribution and quantification of several compounds in specific areas of the eye after instillation.




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