Advanced quantification of drugs and exposure to cells
« Does the active compound reach specific cells or tissue substructures and in what quantity? » are key questions to investigate the potential efficacy in drug discovery. In biomarker discovery, the need to identify which cells express which biomarker is also important to understand the mechanism of action of a disease. To investigate the co-localization between biomarkers and drugs, Pearson Correlation Coefficient (PCC) is usually used but is not sufficient to clearly describe the distribution and concentration levels in cells. We present here different ways to quantify drugs and biomarkers in particular sub-structure of the tissue using latest Multimaging software development.
We present here a new workflow with an updated version of Multimaging, fully compatible with high resolution microscopy with cell recognition and tissue annotation. These tools allow to measure drug and biomarker into cells that avoid misinterpretation of target exposure, pharmacokinetics and pharmacodynamics and conduct to a higher level of confidence. »