biomarker-mapping-skin

Biomarker Mapping on Skin Tape Strips Using MALDI Mass Spectrometry Imaging

Hochart G. (1), Bonnel D. (1), Stauber J. (2), Stamatas G. (3)
1) ImaBiotech SAS, Parc Eurasanté, 152 rue du Dr Yersin, 59120, Loos, France
2) ImaBiotech Corp, 44 Manning Rd, MA, 01821, Billerica, US.
3) Johnson & Johnson Santé Beauté France, 1 rue Camille Desmoulins, 92130, Issy-les-Moulineaux, France

Abstract
Keratinocyte organization and bio-chemistry are important in forming the skin’s pro-tective barrier. Intrinsic and extrinsic factors canaffect skin barrier function at the cellular and mo-lecular levels. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging,
a technique which combines both molecular as-pects and histological details, has proven to be a valuable method in various disciplines including pharmacology, dermatology and cosmetology. It
typically requires ex vivo samples, prepared following frozen tissue sectioning. This paper demonstrates thefeasibility of performing MALDI analysis on tape strips collected non-invasively on skin. The aim is to obtainmolecular imaging of corneocytes on tapes towards novel biological insights. Tapes were collected from two skin sites (volar forearm and cheek) of human volunteers. Ten molecules relating to skin barrier function were detected with a single mode of acquisition at high spatial resolution with a 7 T MALDI-Fourier transform ion cyclotron resonance (FTICR) instrument. The method sensitivity was adequate to create molecular maps which could be overlaid on transmission microscopy images of the same area of the tape. Analysis of the molecular distributions from tapes at the two skin sites was consistent with the known skin properties of the two sites, confirming the validity of the observations. Hierarchical clustering analysis was used to differentiate corneocyte populations based on their molecular profiles. Furthermore, morphological analysis provided a new way of considering statistical populations of corneocytes on the same tape, rather than measuring a single averaged value, providing additional useful information relating to their structure-function relationship.

Keywords
Molecular mapping, Corneocytes, MALDI-FTICR mass spectrometry imaging, Skin barrier

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